Table 2.

Activation of PKC by TGFβ-1

	1		2		3
Experiment	Con	TGFβ1	Con	TGFβ1	Con	TGFβ1
Substrate	0.20	3.1	0.21	3.10	0.38	1.26
+ Activator	2.57	2.5	2.67	2.60	0.97	1.15

Nerve p9, exposed to recombinant vertebrate TGFβ1 for 30 min, and the contralateral control p9 (Con), were homogenized separately and extracts were assayed for PKC activity by measuring the incorporation of ³²P from γ-labeled ATP into a peptide substrate containing a PKC phosphorylation site (see Materials and Methods). Incorporation was assessed by scintillation and ranged from 8,000 to 100,000 cpm depending on the specific activity of the ATP. PKC activity is expressed as pmoles of ³²P incorporated into the peptide/min per μg protein. Phorbol ester and PS (activator) were added to a duplicate tube in each experiment to maximally activate the PKC in the sample.