Figure 2.

The effect of BDNF on glutamate-activated current can be reduced by an NR2B antagonist. (A) Superimposed example traces showing currents during the baseline period and after 20 min exposure to BDNF. Ifenprodil (3 μm) was present in the bath throughout the recording. BDNF elicited a modest increase in current. (B) Time course of the response to BDNF in the presence of ifenprodil (n = 4). The increase in the current was indistinguishable from that seen when AP-5 was used (cf. with Fig. 1D). (C) Superimposed example traces showing the block produced by AP-5 on the component of current increased by BDNF. (D) Time course of the response to BDNF followed by application of AP-5 at 25 min into the recording. Note that AP-5 almost entirely blocked the current that had been increased by BDNF.