Table 3.
Fibroblast ECM array in serum free control and 10 ng/ml TGF-β stimulated fibroblasts
| Serum Free | TGF-β | p Value | |
|---|---|---|---|
| ECM/Growth Factors | |||
| Col1a1 | 4.842 ± 1.399 | 9.614 ± 3.324 | 0.028 |
| Col5a1 | 0.301 ± 0.085 | 0.605 ± 0.230 | 0.038 |
| Fbln1 | 0.012 ± 0.005 | 0.008 ± 0.004 | 0.032 |
| Sparc | 2.709 ± 0.204 | 4.932 ± 0.379 | <0.001 |
| Tgfbi | 0.022 ± 0.016 | 0.013 ± 0.015 | 0.009 |
| Cell Adhesion Molecules | |||
| Ncam1 | 0.043 ± 0.016 | 0.169 ± 0.054 | 0.011 |
| Pecam1 | 0.000060 ± 0.000012 | 0.000022 ± 0.000011 | 0.015 |
| Sgce | 0.187 ± 0.035 | 0.146 ± 0.018 | 0.030 |
| Vcam1 | 0.301 ± 0.136 | 0.161 ± 0.088 | 0.031 |
| MMPs/TIMPs | |||
| Mmp7 | 0.0000075 ± 0.0000011 | 0.0000056 ± 0.0000004 | 0.026 |
| Timp1 | 0.052 ± 0.022 | 0.272 ± 0.136 | 0.035 |
| Timp2 | 0.326 ± 0.109 | 0.188 ± 0.050 | 0.027 |
Data are Avg ± SEM levels (2-ΔCT) normalized to 5 housekeeping genes for n = 4 paired fibroblast sets.