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. 2011 Jun 10;88(6):827–838. doi: 10.1016/j.ajhg.2011.05.008

Figure 5.

Figure 5

FYCO1 Expression in the Lens and Human Lens Cell Culture

(A) RT-PCR amplification of Fyco1 mRNA from P3W mouse eye lens. RT (+) and RT (–) denote controls with or without reverse transcription, respectively. Lane 1, full-length Fyco1 transcript; lane 2, negative control for Fyco1 transcript; lane 3, Gapdh transcript; lane 4, negative control for Gapdh transcript. MWM stands for molecular-weight marker.

(B) Relative expression of Fyco1 in mouse eye lens tissues at various ages. Expression of Fyco1 was measured in lens tissues by qRT-PCR at different time points during aging. Data represent the mean (±SD) on an arbitrary scale (y axis) representing expression relative to the housekeeping gene Gapdh.

(C) Characterization of mutant and wild-type GFP-FYCO1 by immunoblot analysis. Lane 1, transfected wild-type FYCO1-GFP lysate; lane 2, lysate transfected with mutant FYCO1-GFP (p.Leu1376Pro); lane 3, lysate transfected with mutant FYCO1-GFP (p.Leu1288TrpfsX37); lane 4, lysate transfected with mutant FYCO1-GFP (p.Gln736X); lane 5, untransfected lysate. “M” indicates molecular-weight positions of the SeeBlue2 Plus molecular-weight marker. Wild-type proteins migrate at the predicted molecular weight of 197 kDa. Missense mutant proteins migrate at the predicted molecular weight of 197 kDa. Duplication mutant proteins migrate at the predicted molecular weight of 165 kDa. Nonsense mutant proteins migrate at the predicted molecular weight of 105 kDa.