Skip to main content
. 2011 May 13;8:35. doi: 10.1186/1742-4690-8-35

Figure 4.

Figure 4

Pseudotype virus particles bearing the Env protein from other cohort isolates exhibit binding properties equivalent to, or significantly greater than, FeLV-945. A. Sequence comparison of SU proteins from prototype FeLV-A isolates FeLV-A/61E [GenBank:AAA93093], FeLV-A/3281 [GenBank:AAA43051] and FeLV-A/Glasgow [GenBank:AAA43053], from FeLV-945 [GenBank:AAT76450] and from other representatives of the cohort. FeLV-922 [GenBank:AAT76452], FeLV-1046A [GenBank:AAT76457] and FeLV-1049 [GenBank:AAT76458] were isolated from multicentric lymphomas. FeLV-1306 [GenBank:AAT76463] was isolated from myeloproliferative disease. Indicated is the complete amino acid sequence of the mature SU protein encoded by each isolate. The sequence encoded by FeLV-A/61E is shown, identity to FeLV-A/61E is indicated (-), as are amino acid substitutions by one-letter code. The positions of previously identified functional domains VRA, VRB and PRR are underlined. Asterisks indicate positions of the regions used to create substitution mutants shown in Figure 6A and described in the text. B. Flow cytometric binding assays were performed as in Figure 1 except using equivalent titers of pseudotyped viral particles bearing the envelope proteins (Env) of FeLV-945, FeLV-922, FeLV-1049, FeLV-1306, or FeLV-1046A. The geometric mean fluorescence from individual assays is shown, as is the mean of three independent replicate experiments (horizontal bars). Asterisk indicates statistical significance (*; p < 0.001).