Table 1.
Summary of comparative flow cytometric binding assays performed using soluble SU proteins of FeLV-A/61E, 61E/945-VRB or mutant FeLV-A/61E SU proteins substituted of specific amino acids within and surrounding the consensus VRB domain of FeLV-945
| Soluble SU Proteina | Average GMF of Replicate Binding Assaysb | Comparable Binding Phenotype |
|---|---|---|
| FeLV-A/61E | 12.49 | N/A |
| 61E/945-VRB | 22.67 | N/A |
| VRB3aa | 8.64 | FeLV-A/61E |
| N147S | 12.16 | FeLV-A/61E |
| K128N/S130T | 11.92 | FeLV-A/61E |
| I156V/K164R | 10.09 | FeLV-A/61E |
| K128N/S130T/I186V | 22.65 | 61E/945-VRB |
| I156V/K164R/I186V | 21.69 | 61E/945-VRB |
aComparative flow cytometric binding assays were performed using FeLV-A/61E, 61E/945-VRB, or mutated SU proteins named according to the individual FeLV-945 amino acid residues substituted into FeLV-A/61E as shown in Figure 7C. Binding assays were performed using equivalent mass amounts of each SU protein and feline 3201 cells.
bThe average geometric mean fluorescence (GMF) is shown for three (N147S), four (VRB3aa, I156V/K164R, K128N/S130T, I156V/K164R/I186V), or five (K128N/S130T/I186V) replicate binding assays using three or four independently generated and titered batches of SU proteins.