Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Med. 2011 May 22;17(6):684–691. doi: 10.1038/nm.2388

Figure 2.

Figure 2

Effect of conditionally activating NeoR-containing HBM Lrp5 alleles. Graphs depicting (a) femoral and vertebral trabecular BV/TV in WT mice and in mice with NeoR-containing HBM Lrp5 alleles. (b) femoral trabecular BV/TV and (c) vertebral trabecular BV/TV in mice with (shaded bars) and without (unshaded bars) inherited NeoR-containing HBM Lrp5 alleles (+/AN or +/GN, and +/A or +G, respectively), and with and without Villin::Cre (+V and −V, respectively) or Dmp1::Cre (+D and −D, respectively) transgenes. (d) Photographs of agarose gels containing PCR amplimers derived from mouse genomic DNA extracted from either duodenum or femur cortex of mice with different Lrp5 and Cre-transgene genotypes. PCR amplimers correspond to the sizes depicted in Fig. 1a. (top) amplimers from the Dmp1::Cre cross. (bottom) amplimers from the Villin::Cre cross. WT allele (arrowhead), AN or GN allele (double arrowhead), A or G allele (arrow). (e) Graphs depicting fluorochrome-derived bone formation parameters in the distal femur from 9-wk-old female mice administered double calcein labeling. X-axis group notations follow those described for panel b. (f) Graphs depicting the proportion of distal femur trabecular bone surface covered by osteoclasts (left) and osteoblasts (right). (g) Graphs depicting femur and vertebra trabecular BV/TV in 12-wk-old HBM Lrp5 mice with (shaded bars) and without (unshaded bars) inherited NeoR-containing alleles (+/AN and +/A), respectively, and with and without the Prx1::Cre transgene (+P and −P, respectively). MS/BS, MAR, BFR/BS are as defined in Fig. 1. The numbers of mice studied (n =) are indicated, as are error bars equal to 1 s.d. Asterisks (*) indicate a significant difference (p < 0.05) when compared to NeoR-containing littermates that did not inherit a Cre-transgene.