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. 2010 Nov;89(11):1293–1298. doi: 10.1177/0022034510378427

Figure 2.

Figure 2.

In vitro characterization of mandible vs. long-bone BMSCs. (A) Representative of 5 independent experiments of ALP staining at 3-, 7-, 14-, and 21-day cultures of long-bone (LB) vs. mandible (MB) BMSCs in osteogenic media. (B) Representative of 4 independent experiments of von Kossa staining at 14- and 21-day cultures of long-bone (LB) vs. mandible (MB) BMSCs in osteogenic media. (C) ALP activity assay at 3- and 7-day cultures of long-bone (LB) vs. mandible (MB) BMSCs in osteogenic media (average of 3 independent experiments). (D) [45]Ca assay at 21 days’ culture of long-bone (LB) vs. mandible (MB) BMSCs in osteogenic media (average of 3 independent experiments). (E) ALP and (F) OCN mRNA expression determined by qPCR of long-bone (LB) vs. mandible (MB) BMSCs cultured in osteogenic media for 3 and 7 days (average of 4 independent experiments). *p < 0.05; error bars represent standard error of the mean.