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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Expert Rev Proteomics. 2011 Feb;8(1):43–59. doi: 10.1586/epr.10.109

Figure 2. Mass shift perturbation data between singly and doubly ligated microtubules.

Figure 2

Mass shift perturbation data from analyses of doubly ligated microtubules relative to the singly ligated state. (A) Scatterplot of replication membrane scaffold protein data for a comparison of doubly ligated microtubules with laulimalide-stabilized microtubules, highlighting the perturbations unique to docetaxel. (B) Significant changes in (A) mapped to their locations in tubulin sequence. (C) Scatterplot of replication membrane scaffold protein data for a comparison of doubly ligated microtubules with docetaxel-stabilized microtubules, highlighting the perturbations unique to laulimalide. (D) Significant changes in (C) mapped to their locations in tubulin sequence. Each point in (A,C) represents the shift perturbation of a single peptide in millimass units. The horizontal dotted lines represent a ±2 standard deviation cutoff based on noise in ΔD measurements and the vertical dotted line represents a 1-p cutoff value of 0.95. Green represents positive mass shifts resulting from single ligation and red represents negative mass shifts resulting from single ligation. The sequence maps (B,D) are arranged with α-tubulin on the bottom and β-tubulin on the top.

mmu: Millimass unit.

Reproduced with permission from [71]. For more details, refer to the text or reference [71].