Figure 2. Mass shift perturbation data between singly and doubly ligated microtubules.

Mass shift perturbation data from analyses of doubly ligated microtubules relative to the singly ligated state. (A) Scatterplot of replication membrane scaffold protein data for a comparison of doubly ligated microtubules with laulimalide-stabilized microtubules, highlighting the perturbations unique to docetaxel. (B) Significant changes in (A) mapped to their locations in tubulin sequence. (C) Scatterplot of replication membrane scaffold protein data for a comparison of doubly ligated microtubules with docetaxel-stabilized microtubules, highlighting the perturbations unique to laulimalide. (D) Significant changes in (C) mapped to their locations in tubulin sequence. Each point in (A,C) represents the shift perturbation of a single peptide in millimass units. The horizontal dotted lines represent a ±2 standard deviation cutoff based on noise in ΔD measurements and the vertical dotted line represents a 1-p cutoff value of 0.95. Green represents positive mass shifts resulting from single ligation and red represents negative mass shifts resulting from single ligation. The sequence maps (B,D) are arranged with α-tubulin on the bottom and β-tubulin on the top.

mmu: Millimass unit.

Reproduced with permission from [71]. For more details, refer to the text or reference [71].