Fig. 2.

All three β-ARs are expressed in human detrusor. These samples of normal bladder were obtained from older (80-year-old (a,b), 66-year-old (c)) females, versus young (34-year-old (d,e), 38-year-old (f)) females and stained for expression of the β₁-(a,d), β₂ -(b,e), and β₃-(c,f) ARs with the SC-567 (a,d) LS-A2662 (b,e), and LS-A4198 (c,f) antibodies, respectively. Panels g,h demonstrate an absence of staining obtained in the presence of non-immune rabbit IgG antibody for the corresponding tissues (66- and 38-year-old female samples for the β₃ antibody, respectively). Similar negative results were obtained in the absence of primary antibody on the samples for the β₁- and β₂-AR antibodies (data not shown). Blush to faint staining was observed in the muscularis propria for the β₁ receptor, and was similar in the younger and older samples. The muscularis propria was faint to moderately positive for β₂ -ARs in both the younger and older samples. A similar faint expression of the β₃-AR was observed in the muscularis propria in all samples regardless of age. The antibodies were used at 2.5, 10, and 10 μg/ml, respectively