Fig. 8.

Quantitative image analysis of the urothelial β-AR expression as a function of age. The density of expression of the β₁-, β₂-, and β₃-ARs was quantified after staining with the SC-567, SC-9042, and LS-A4198 antibodies as described in “Materials and methods”. Each data point represents the average of three density measurements for the area comprising all urothelial cell layers for a single individual. No clear correlation is observed between expression of either the β₁-(a SC-567) or β₃- (c LS-A4198) ARs with age. However, the data indicate a non-linear decreasing density in β₂-AR expression with age only in the urothelium from the trigone region (b SC-9042 antibody). Similar results were obtained when the data were quantitated after staining for the β₂-AR with the LS-A2662 antibody (data not shown)