Table 4.

Comparison of specific δG° (ΔG° for one step of DNA helix) predicted from the K_M and the energy parameters of ds ODNs

ds ODN ID	Predicted	Calculated or measureda					Predicted
	δG°b (kcal/mol/step)	ΔG° (kcal/mol)	ΔH° (kcal/mol)	ΔS° [cal/(mol × K)]	Tm (°C)	kcata (min−1)	Δkcatc (min−1)
ODN1	−1.68	−19.6	−138.47	383	64.0	1.00	0.02
ODN2	−1.71	−18.4	−113.59	307	66.7	1.40	0.06
ODN3	−1.75	−20.7	−129.05	349	69.3	1.10	−0.10
ODN4	−1.72	−21.2	−139.77	382	67.9	0.90	0.07
ODN5	−1.63	−19.4	−139.68	388	63.1	1.10	−0.02
ODN6	−1.88	−20.7	−121.12	324	71.8	1.20	−0.23
ODN7	−1.36	−14.6	−146.86	426	50.5	1.30	−0.35
ODN8	−1.23	−11.6	−108.02	311	46.0	0.96	−0.47
ODN9	−1.89	−21.1	−137.78	376	68.2	2.90	0.68
ODN10	−1.50	−16.7	−136.68	387	56.7	1.00	0.44
ODN11	−2.12	−19.9	−91.50	231	81.3	2.40	−0.05
ODN12	−1.61	−17.9	−123.44	340	62.5	1.20	0.00
ODN13	−1.61	−16.0	−129.44	366	56.0	0.94	−0.04
ODN14	−1.23	−7.6	−106.92	320	34.2	1.30	−0.13
ODN15	−1.23	−3.7	−102.34	318	23.1	0.40	−0.16
ODN16	−1.36	−2.2	−101.60	320	18.9	0.48	−0.08
ODN17	−1.23	−8.7	−137.07	414	37.5	1.70	0.27
ODN18	−1.68	−16.8	−120.95	336	59.9	0.80	−0.18
ODN19	−1.68	−14.6	−109.83	307	55.2	1.90	0.92
ODN20	−1.68	−18.6	−140.74	394	61.0	1.40	0.57
ODN21	−1.63	−13.8	−94.70	261	55.3	1.40	0.42
R		0.813d	0.027	0.270	0.880	−0.517	−0.282
P		<10−5	>0.9	>0.2	<10−6	<0.025	>0.2

^ak_cat from Table 2, other parameters calculated using the ACTIVITY software.

^bFrom Equation (10).

^cΔk_cat = k_cat – k_cat{e⁰–₁₀…G…e⁰+₁₀/e^#_–10…C…e^#₊₁₀}.

^dR, correlation coefficient; P, level of significance.