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. 2011 Feb 9;39(11):4756–4768. doi: 10.1093/nar/gkr038

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — hMTr1 and hMTr2 act on the U1 and U2 snRNAs in vitro. In vitro transcribed U1 and U2 snRNA molecules with ³²P-labeled cap0 (A and C) or cap01 (B and D) structures were incubated with one of the indicated enzymes, added in the order indicated and SAM. After every modification step RNA was purified by phenol/chloroform extraction and ethanol precipitation. Final products were fragmented with nuclease P1 (A and C) or RNase T2 (B and D). Digestion products were resolved on 21% polyacrylamide/8 M urea gels and visualized by autoradiography. Cap structures modified with VMT (A and C) or TbMTr2 (B and D) were used as positive controls. Asterisks indicate positions of ³²P-labeled phosphates.