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. 2011 Feb 8;39(11):4851–4865. doi: 10.1093/nar/gkr045

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Influence of nPTB on hydroxyl radical cleavage of the EMCV IRES in IRES/eIF4Gm/eIF4A complexes from Fe(II)-tethered eIF4Gm. (A) Ribbon diagram of the HEAT-1 domain of eIF4G (PDB: 1hu3), with spheres indicating newly introduced cysteines. (B–D) Primer extension analysis of hydroxyl radical cleavage of the IRES from Fe(II) tethered eIF4Gm in IRES/eIF4Gm/eIF4A complexes in the presence/absence of nPTB. Lanes C, T, A, G depict the corresponding EMCV sequence. IRES domains and nucleotides are indicated on the left of each panel, and cleavage sites are shown on the right; boxed numbers indicate sites at which nPTB enhanced cleavage.