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. 2011 Feb 9;39(11):4653–4663. doi: 10.1093/nar/gkr055

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Genetic organization of restriction-modification system Eco29kI. The eco29kI genes are schematically shown by colored arrows, with arrow direction matching the direction of transcription. The DNA sequences of parts of the eco29kIR gene that include eco29kIR and AS promoters, and the eco29kIM promoter are expanded, respectively, above and below the eco29kI genes schematics (both DNA strands are shown). The initiating codons of both eco29kI genes are highlighted. The Res_P1, Res_P2 and Meth_P start sites are indicated by rightward arrows above the sequence. The AS_P1 and AS_P2 start sites are indicated by leftward arrows below the sequence. Experimentally defined −10-boxes are labeled and over- or underlined. Nucleotide positions substituted in engineered down-mutations discussed in the text are marked with ‘asterisks’.