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. 2011 Feb 9;39(11):4653–4663. doi: 10.1093/nar/gkr055

Figure 2.

Figure 2.

Mapping eco29kIR gene promoters. (A) RNA was purified from E. coli cells harboring a plasmid with the beginning as well as upstream eco29kIR sequence fused to promoterless galK and subjected to primer extension reaction with a galK-specific primer. (B) Overnight growth of E. coli cells harboring plasmids with eco29kIR promoters fused to promoterless galK on a McConkey agar plate. The following plasmids were used: pR-galK—a DNA fragment (from −72 to +34) of eco29kIR fused to galK; pRmut1-galK—pR-galK with inactive Res_P1; pRmut2-galK—pR-galK with inactive Res_P2; pRmut3-galK—pR-galK with inactive Res_P1 and Res_P2.