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. 2011 Feb 9;39(11):4653–4663. doi: 10.1093/nar/gkr055

Figure 3.

Figure 3.

Mapping the eco29kIM gene promoter. (A) Overnight growth of E. coli cells harboring plasmids with internal eco29kIR fragments fused to promoterless galK on a McConkey agar plate. The following plasmids were used: pM-galK—a fragment (from +408 to +472) of eco29kIR fused to galK; pMmut1-galK—pM-galK with inactive Met_P; pUTR-galK—a fragment (from +469 to +682) of eco29kIR fused to galK; pMUTR-galK—a fragment (from +408 to +682) of eco29kIR fused to galK. (B) Results of primer extension analysis carried out with RNA purified from cells harboring a plasmid with eco29kIM promoter fused to promoterless galK are presented.