Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Feb 9;39(11):4653–4663. doi: 10.1093/nar/gkr055

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 7. — In vitro transcription and promoter complex formation on eco29kIR and AS promoters. (A) Escherichia coli RNAP σ⁷⁰ holoenzyme was combined with Eco29kI DNA fragments carrying indicated promoters and abortive transcription initiation reactions with dinucleotide monophosphate primers specific for Res_P1 (GpA) or Res_P2 (CpU) and ³²P UTP were performed. Reaction products (GpApU and CpUpU for Res_P1 and Res_P2, respectively, bold-type face denoting ³²P phosphate) were resolved by denaturing PAGE and revealed using a Phosporimager. (B) Single round transcription using wild-type template Eco29kI DNA (R + AS) and a template DNA with inactive AS promoters (R − AS).