Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Feb 9;39(11):4653–4663. doi: 10.1093/nar/gkr055

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 8. — Downstream translation initiation abolished the inhibitory effect of AS transcripts. The scheme at the top shows two kinds of eco29kIR promoter lacZ fusions studied. Relative abundance of lacZ transcripts measured with qRT–PCR in cells harboring the following plasmids: pRAS-lacZ—an eco29kIR fragment (from −44 to +282) cloned into pLacZ; pRASmut3-lacZ—pRAS-lacZ with inactive AS promoters; pRAS_SD-lacZ—an eco29kIR fragment (from −44 to +282) cloned into pLacZ_SD; pRASmut3_SD-lacZ—pRAS_SD-lacZ with inactive AS promoters is shown below. The amount of cat transcript revealed with appropriate primers was used to normalize lacZ transcript abundance in each reaction mix.