Figure 3.

Loss of hfq or SMc01113 similarly down-regulates target transcript accumulation. WT and the smc01113 and the hfq mutants were grown in the RMM till exponential (ppiA, katB and cysK1) or stationary phase (sodC, agpA, livK, ehuB and atpD), RNA was extracted and qPCR was performed. Transcript accumulation in the WT strain was fixed to 1 and relative transcript abundance to WT in the two mutants was evaluated. ‘Single’ and ‘double asterisk’ shows significant difference at P ≤ 0.05 and P ≤ 0.01, respectively.