Figure 5.

Loss of hfq or SMc01113 similarly deregulates sRNA accumulation. The WT, smc01113 and hfq mutants, were grown in the RMM till exponential (sras03, 11, 16, 45, 47, 51 and 65) or stationary phase (sra35 and sra63), RNA was extracted and qPCR was performed. Transcript accumulation in the WT strain was fixed to 1 and relative transcript abundance to WT in the two mutants were evaluated as in Figures 3 and 4. ‘Single’ and ‘double asterisk’ shows significant difference at P ≤ 0.05 and P ≤ 0.01, respectively.