Table 4.

Summary of polymorphisms found in our parent strain, yMWB3-15, by Solexa sequencing and the S. pombe reference genome, yFS101 (972h-)

Chr	Position	SangerPos	Ref	WT	Cover	Gene	Name	Mutation	FS101	MWB315
1	733352	733352	G	A	66	SPAC22G7.04	pan2	e	G	A
1	853328	853328	T	G	80	SPAC23G3.01	rpb2	L872R	T	G
1	877349	877349	C	T	71	SPAC23G3.06	nop58	N98a	C	T
1	943745	943745	T	C	48	SPAC1687.22c	puf3	I709M	T	C
1	1E + 06	1263527	A	G	58			*	A	G
1	2E + 06	1664907	T	A	42			*	T	A
1	2E + 06	2207525	G	T	51	SPAC1327.01c	(txn fctr)	L191I	G	T
1	2E + 06	2277237	C	A	63	SPAC4G9.12	(glco kinase)	Y13stop	C	A
1	2E + 06	2283617	T	C	42			*	T	C
1	3E + 06	2643596	C	A	67			*	C	A
1	3E + 06	2747078	A	G	49	SPAC6F6.08c	cdc16	c	A	G
1	4E + 06	3548913	T	A	32			*	T	A
1	6E + 06	5543973	T	C	46			*	T	C
2	535941	518160	G	A	43			*	G	A
2	683208	665427	T	C	49			*	T	C
2	998064	980283	T	C	46	SPBP35G2.08c	air1	S81a	T	C
2	1E + 06	990639	C	T	87	SPBP35G2.13c	swc2	S243a	C	T
2	1E + 06	1422317	T	A	42	SPBC691.02c	tip20	L531a	T	A
2	2E + 06	1553976	A	T	23			*	A	T
2	2E + 06	2007715	A	G	40			*	A	G
2	3E + 06	2806053	G	A	42			*	G	A
2	3E + 06	2901509	G	T	47		miscRNA		T	T
3	1E + 06	1316977	T	A	80	SPCC1322.13	ade6d	C215stop	NA	A
3	2E + 06	1647827	G	T	33			*	G	T
3	2E + 06	1862803	A	G	48			*	A	G
Mt	1788	1788	C	T	9483		rRNA		T	T
Mt	2091	2091	A	G	7144		rRNA		G	G
Mt	4102	4102	C	G	7556		rRNA		G	G
Mt	4463	4463	C	A	7275		rRNA		A	A
Mt	6906	6906	A	T	6332	CDS		S21C	T	T
Mt	8529	8529	G	T	7585	cox1	cox1	E451D	T	T
Mt	10309	10309	T	C	5261	cob1	cob1	I46T	C	C
Mt	11218	11218	T	A	9344	aic ORF		I121N	A	A
Mt	11644	11644	A	C	6613	aic ORF		H263N	C	C
Mt	13196	13196	C	T	7841	aic ORF		a	T	T
Mt	13751	13751	C	T	6789	cob1	cob1	a	T	T
Mt	15082	15082	A	–	6204	atp6	atp6	L109Ff	–	–
Mt	[15088]	[15088]	–	A	5950	atp6	atp6	R111Gf	A	A
Mt	15200	15200	G	T	6428	atp6	atp6	V149F	T	T
Mt	18648	18648	G	A	5716	cox2	cox2	V301I	A	A
Mt	19152	19152	A	G	5906	cox2	cox2	S198G	G	G

Chr = chromosome, 1,2,3 or mitochondria (Mt). Cover = coverage.

Resulting coding change, if any is noted in the ‘Mutation' column, *^-intergenic, ^a-synonymous mutation in coding region, ^c-intronic, ^d-ade6-704 allele mutation confirming previous sequencing (65), ^e-intronic based on revised splice prediction (72), ^f-atp6 frameshift mutations result in two amino acid mutations leaving 110 unchanged.

Each nuclear SNP was verified by conventional PCR-mediated sequencing and the identities are noted in the rightmost columns.