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. 2011 Feb 14;39(11):4866–4874. doi: 10.1093/nar/gkr073

Table 1.

Threonylation by ScMST1

Anticodon loop kcat (min−1) Km (μM) kcat/Km (μM min−1) Relative kcat/Km
Inline graphic
    WT UUUUAGGU 2.8 ± 0.4 0.29 ± 0.09 10.3 ± 2.3 100
    G−1 UUUUAGGU 0.094 ± 0.032 1.5 ± 0.6 0.065 ± 0.006 0.63
Inline graphic
    WT UUUGUAA 2.3 ± 0.1 0.44 ± 0.04 5.4 ± 0.6 52
tRNAHis
    WT UUGUGGU ND ND ND ND
    InsA35 UUGUAGGU ND ND ND ND
    InsA35/C73A UUGUAGGU ND ND ND ND
    ΔG−1 UUGUGGU 0.27 ± 0.10 0.74 ± 0.41 0.39 ± 0.06 3.8
    ΔG−1/InsA35 UUGUAGGU 0.34 ± 0.07 0.20 ± 0.02 1.7 ± 0.5 17
    ΔG−1/C73A UUGUGGU 0.23 ± 0.01 0.85 ± 0.32 0.29 ± 0.09 2.8
    ΔG−1/C73A/InsA35 UUGUAGGU 1.2 ± 0.4 0.57 ± 0.26 2.4 ± 1.0 23

ND, not determined due to low activity.