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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Langmuir. 2011 Feb 16;27(6):2739–2746. doi: 10.1021/la104313z

Figure 2.

Figure 2

Scheme for DPP immobilization to a model substrate. (A) The DPP conjugates are formed in two steps including: (i) the addition of maleimide-PNA (blue) to pDNA (black circles) to form a stable triplex followed by (ii) the binding of thiol-terminated peptide (green) to the pDNA-PNA by thiol-maleimide reaction. The gold-coated surface is activated by (B) MUA (orange) treatment of the gold-coated surface to form a SAM with terminal carboxylic acid functionalities. The DPP conjugates are attached to the surface by the (C) reaction of the amine functionality of the N-terminus of the peptide with the surface carboxylic acid in the presence of NHS and EDC to form stable amide bonds between the conjugates and the surface.