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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):3004–3013. doi: 10.1002/jcp.22648

Figure 2. Akt1 deficiency in fibroblasts results in decreased secretion of ECM proteins.

Figure 2

(A) Western analysis of conditioned media secreted by WT and Akt1−/− MEFs for fibronectin and collagen type I. Also shown are the expression levels of fibroectin and β-actin in the corresponding cell lysate. (B) Bar graph showing densitometry analysis of 4 different samples of conditioned media for the secreted fibronectin and collagen type I by WT and Akt1−/− MEFs. (C) Bar graph showing ELISA for secreted fibronectin in the conditioned media secreted by WT and Akt1−/− MEFs. (D) Western analysis of conditioned media secreted by NIH 3T3 fibroblasts transfected with WT-, DN- and myr-Akt1 probed for fibronectin. Also shown are the expression levels of β-actin in the corresponding cell lysates.