Figure 5. Bleomycin-induced Akt activation and secretion of ECM proteins is inhibited by TGF inhibitor SB431542.

NIH3T3 fibroblasts were grown to confluence and were treated with 1mU bleomycin and/or TGFβ inhibitor SB431542), and further incubated at 37°C in a CO2 incubator. After 12 hours, conditioned media was collected and cells were lysed using RIPA buffer with protease and phosphatase inhibitors. Figure shows phosphorylation status of Akt and S6 ribosomal protein as well as secreted fibronectin and collagen type I.