Fig. 7.

ER–mitochondrial Ca²⁺ transfer drives the adaptive metabolic response. (A) Oxygen consumption rates were measured in HeLa cells treated for 4 hours with 0.5 μg/ml tunicamycin (Tun) in combination with 2 μM xestospongin B as indicated. (B) Oxygen consumption rates were measured in HeLa cells treated for 4 hours with 0.5 μg/ml tunicamycin in combination with 10 μM RuRed as indicated. (C) The quantification of dead cells (PI positive) and cells with a low ΔΨ_m [low DiOC₆(3) staining] after 4 hours treatment with 1 μg/ml tunicamycin in combination with 10 μM RuRed was determined by flow cytometry. Data are means±s.e.m. *P<0.05; **P<0.01 compared with untreated controls; ^#P<0.05 compared with tunicamycin alone.