Table 1.

Summary of biological data for zebrafish, medaka, and Xiphophorus males of four species including sperm production per male (Yang and Tiersch, 2011b), and calculation of spem numbers that would be produced by pooling of samples from 20 males for high-throughput processing. Assuming that sperm would be adjusted to a concentration of 5 × 10⁷ cells/mL with a loading volume of 100 μL per 0.25-mL French straw, and used for fertilization of 50-100 egg from one female for zebrafish, or 50-100 eggs from 10 females for Japanese ricefish (medaka), or 1-2 females for Xiphophorus. Pooling would allow quality assessment to be performed on the batched material and would greatly reduce waste compared to sampling from individual males.

	Sample size	Body length (cm)	Body mass (g)	Testis mass (mg)	Sperm number per mg testis (× 106)	Sperm production per male (× 106)	Number of straws (per male)	Number of straw if pooling 20 males
Zebrafish	45	2.4 ± 0.2	0.295 ± 0.066	3.2 ± 2.0	7.7 ± 2.0	24.6	5	98
Medaka	74	2.6 ± 0.2	0.311 ± 0.052	2.0 ± 0.6	2.0 ± 0.4	4.0	1	16
X. helleri	45	3.2 ± 0.3	0.630 ± 0.168	9.2 ± 5.5	5.4 ± 2.2	49.7	10	199
X. couchianus	66	2.0 ± 0.2	0.184 ± 0.052	3.1 ± 1.0	5.4 ± 2.5	16.7	3	67
X. maculatus	117	2.5 ± 0.3	0.474 ± 0.149	7.1 ± 3.7	5.8 ± 2.8	41.2	8	165
X. variatus	35	2.5 ± 0.2	0.298 ± 0.096	6.4 ± 3.4	2.4 ± 1.3	15.4	3	61