Figure 3. Enhanced lipid peroxidation and SIRT3 protein carbonylation in a 6-week chronic ethanol model.
(A) A TBARS assay demonstrates a significant increase in lipid peroxidation in this chronic ethanol model, as quantified by detecting MDA levels. (B) Biotin hydrazide (BH) conjugation displays a significant increase in SIRT3 protein carbonylation in these week 6 mice (Control = 100%, SEM 13.7. Ethanol = 207%, SEM 11.3). (C) Chronic ethanol consumption does not alter SIRT3 protein expression. VDAC was used as the load control.