Table 1.

nLC-MS/MS and MALDI-TOF/TOF MASCOT results from rSIRT3 analysis: Untreated and modified by 4-HNE (NaBH₄ reduced).

AA	Exp	Calc	Sequence	MS/MS
100–120	1970.41	1969.97	RSISFSVGASSVVGSGGSSDK	*
123–133	1255.71	1255.71	LSLQDVAELIR	*
140–158	1901.00	1902.01	VVVMVGAGISTPSGIPDFR	*
140–158	1916.98	1917.00	VVVMVGAGISTPSGIPDFR (1 Met Oxidation)	*
198–214	2110.06	2110.06	ELYPGNYKPNVTHYFLR	*
215–219	624.33	624.36	LLHDK
215–224	1176.74	1176.73	LLHDKGLLLR
225–235	1320.67	1320.67	LYTQNIDGLER	*
236–243	757.43	757.43	VSGIPASK
244–269	2861.40	2861.36	LVEAHGTFASATCTVCQRPFPGEDIR (1 Cys Carb)	*
244–269	2918.38	2918.38	LVEAHGTFASATCTVCQRPFPGEDIR (2 Cys Carb)	*
280–301	2514.29	2514.28	CPVCTGVVKPDIVFFGEPLPQR (2 Cys Carb)	*
280–301	2615.61	2615.39	CPVCTGVVKPDIVFFGEPLPQR (1 Cys 4-HNE [+158], 1 Cys Carb)	*
341–345	627.38	627.41	LLINR
341–356	1868.99	1869.07	LLINRDLVGPLAWHPR
346–356	1259.68	1259.80	DLVGPLAWHPR	*
346–356	1418.04	1417.81	DLVGPLAWHPR (1 His 4-HNE [+158])	*
359–384	2896.45	2896.43	DVAQLGDVVHGVESLVELLGWTEEMR (1 Met Oxidation)
385–389	629.34	629.35	DLVQR
385–393	1044.54	1044.54	DLVQRETGK
390–399	1058.74	1058.52	ETGKLDGPDK	*

Amino acid number (AA), experimental (Exp.) and calculated (Calc.) nominal masses for each peptide are shown.

^*MS/MS analysis confirmation was obtained. (Carb = Carbamidomethyl)