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. 2011 Apr 6;31(14):5414–5425. doi: 10.1523/JNEUROSCI.2456-10.2011

Figure 6.

Figure 6.

NF-κB-dependent increase in spine density involves the novel gene target, PSD-95. A, p65-deficient neurons have significantly decreased levels of PSD-95 at DIV 16 (p = 0.005), but not at DIV 21 (p = 0.09). Representative immunoblot (left) and quantitated data normalized to GAPDH (right). B, Basal expression of PSD-95 mRNA is decreased in DIV 14 p65 deficient neuronal cultures (p65−/−) compared with p65 wild-type cultures (p65+/+) (p = 0.001). C, At DIV 21, p65−/− cultures no longer have significantly lower basal PSD-95 mRNA levels, but fail to increase PSD-95 expression in response to bicuculline (Bic)-induced activation of NF-κB (p = 0.001). D, PSD-95 is required for the rescue of decreased spine density in p65-deficient neurons by p65 expression. DIV 16 pyramidal neurons from RelAF/F mice expressing Cre recombinase show a significant loss of spine density that is rescued by p65 expression in the presence of control mutant siRNA, but not in the presence of siRNA for PSD-95 (Cre/p65/mut.siPSD95, n = 8, p = 0.037; Cre/p65/siPSD95, n = 13). Data are means ± SEM. See also supplemental Figure S4 (available at www.jneurosci.org as supplemental material).