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Aciculatin suppresses JNK, p38 MAPKs and MKK4, MKK3/6 phosphorylation in LPS-activated macrophages. RAW264.7 cells (1 × 106) were treated with (A) 1 μg/mL LPS for the indicated time periods or (B, C) 1 μg/mL LPS with or without aciculatin (1-10 μM) for 30 min, and cell lysates were then subjected to western blot analysis for the indicated proteins.
