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. 2011 May 7;30(1):51. doi: 10.1186/1756-9966-30-51

Figure 4.

Figure 4

The expression of Akt mRNA, Akt protein and p-Akt protein in Jurkat and Hut cells after CCL21 co-culture in vitro. RT-PCR amplication and Western Blot analysis of the two cell lines under the different concentration of CCL21. β-actin is positive control in RT-PCR amplication and GAPDH is positive control in Western Blot analysis. The relative grey scale of Akt mRNA, Akt protein and p-Akt protein in Hut cell were all higher than that in Jurkat cell with corresponding concentration of CCL21.