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. 2011 May 19;30(1):60. doi: 10.1186/1756-9966-30-60

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Copyright ©2011 Jiang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Restrictive enzyme incision analysis for pGensil-1/MTA1 shRNA plasmid using RT-PCR. M: DNA Marker. lane 1: pGenesil-1/MTA1 shRNA(pGM1) plasmid was cut off by BamHI and HindIII. lane 2: pGenesil-1/MTA1 shRNA(pGM1) plasmid was cut off by BamHI and XbaI.lane 3: pGenesil-1/MTA1 shRNA(pGM1) plasmid was cut off by HindIII and XbaI. lane 4: pGenesil-1/MTA1 shRNA(pGM2) plasmid was cut off by BamHI and HindIII. lane 5: pGenesil-1/MTA1 shRNA(pGM2) plasmid was cut off by BamHI and XbaI. lane 6: pGenesil-1/MTA1 shRNA(pGM2) plasmid was cut off by HindIII and XbaI.