Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 15;22(12):1960–1970. doi: 10.1091/mbc.E11-01-0053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Gómez-Nicola et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — IL-15 is expressed in neurospheres during proliferation and differentiation. (A–F) Immunocytochemical analysis of the expression of IL-15 (red; A, C, D, F) in nestin-positive (green; B, C) or DCX-positive (green; E, F) cells. Magnifications are shown in the right-hand inset, indicating colocalization with white arrowheads. Nuclei are stained with Hoechst (blue). Neurospheres were evaluated with confocal microscopy. Scale bar in A–F, 20 μm (shown in F). (G) RT-PCR analysis of IL-15 mRNA expression under proliferative culture conditions (+EGF, +FGF) at 24, 48, and 72 h. Resting neurospheres (without EGF or FGF) were used as control (CTL). GAPDH expression was used as housekeeping gene. (H) RT-PCR analysis of IL-15 mRNA expression under differentiation culture conditions (+2% FBS) at 4, 7, and 10 d. Proliferative neurospheres (+EGF, +FGF) were used as control. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression was used as housekeeping gene.