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. 2011 Jun 15;22(12):1960–1970. doi: 10.1091/mbc.E11-01-0053

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© 2011 Gómez-Nicola et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — IL-15 regulates neurosphere differentiation state. (A) Western blotting analysis of the expression of markers of astrocytes (GFAP), neurons (βIII tubulin), oligodendrocytes (MBP), and neural progenitor cells (DCX) in WT and IL-15 knockout NSCs cultured in differentiating conditions (2% FBS; poly-l-lysine) for 4, 7, or 10 d. (B) Immunocytochemical analysis of the cellular proportions during differentiation of NSCs of WT or IL-15 KO cells. Cells were cultured in differentiating medium (2% FBS; poly-l-lysine) for 4, 7, or 10 d and analyzed for the percent of neurons (βIII tubulin+; black bars), astrocytes (GFAP+; white bars), or oligodendrocytes (O4+; gray bars), expressing data as mean ± SEM of positive cells. Statistical differences of WT vs. IL-15−/−: *p < 0.05. Data were analyzed with an ANOVA and a post hoc Tukey test.