FIGURE 11:

Pab1^17A but not Pab1^20A toxicity is suppressed by globally lowering mRNA levels. (A) Tenfold serial dilutions of cells were spotted onto medium containing glucose (expression repressed) or galactose (expression induced) to measure spotting efficiency and toxicity of Pab1^17A-GFP or Pab1^20A-GFP in wild-type and RNA PolII mutant (rpb2–10) cells. (B) Wild-type and rpb2–10 cells expressing Pab1^8A-GFP, Pab1^17A-GFP, or Pab1^20A-GFP were assayed for presence of GFP-positive inclusions and morphology defects after 16-h induction and reported as a percentage of GFP-positive cells. More than 150 cells in three independent cultures were counted for each. Bars are SD. *p < 0.05 by Student's t test when Pab1^polyA rpb2–10 strains were compared with corresponding RPB2 strain. (C–E) As in (A) except that cells expressing Htt^25Q or Htt^103Q (C), TDP-43-GFP (D), or α-syn (E) were assayed.