FIGURE 2:

Expression levels of endogenous and PolyA-expanded Pab1. (A) Tenfold serial dilutions of cells were spotted onto medium containing glucose (polyA-expanded Pab1 expression repressed) or varying concentrations of galactose (polyA-expanded Pab1 expression induced) to measure spotting efficiency and toxicity of Pab1 polyA-expansions at increasing Pab1 expression levels. (B) Immunoblot with anti-HSV to detect Pab1 tagged at its native locus (endo) and Pab1 polyA-expansion variants grown in increasing galactose concentrations. Equal OD equivalents were loaded on the polyacrylamide gel.