FIGURE 9:

Pab1^17A but not Pab1^20A toxicity is suppressed in mRNA export mutants. (A) Tenfold serial dilutions of cells were spotted onto medium containing glucose (expression repressed) r galactose (expression induced) to measure spotting efficiency toxicity of Pab1^8A-GFP, Pab1^17A-GFP, or Pab1^20A-GFP in wild-type and deletion mutant cells. (B) Wild-type and deletion mutant cells expressing Pab1^8A-GFP, Pab1^17A-GFP, or Pab1^20A-GFP were assayed for presence of GFP-positive inclusions and morphology defects after 16-h induction and reported as a percentage of GFP-positive cells. More than 150 cells in three independent cultures were counted for each. Bars are SD. *p < 0.05 by Student's t test when polyA-expansion Pab1 mutant strains were compared with corresponding wild type.