TABLE 3:
Antibodies and probes.
| Antibodies/probes | Dilution | Source |
|---|---|---|
| Anti–DE-cad2 | 1:100 | Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA (DSHB) |
| Anti-zipper (myosin II heavy chain) | 1:1000 | D. Kiehart (Duke University, Durham, NC) |
| Anti-Armadillo (N27A1) | 1:100 | DSHB |
| Anti-Cno | 1:1000 | J. Sawyer and N. Harris (University of North Carolina at Chapel Hill) |
| Anti-Cor | 1:10000 | R. Fehon (University of Chicago, Chicago, IL) |
| Anti-Verm | 1:300 | S. Lushnig (Universität Zürich, Zürich, Switzerland) |
| Anti-DSRF | 1:500 | S. Blair (University of Wisconsin, Madison, WI) |
| Anti-Dys | 1:800 | L. Jiang (Oakland University, Rochester Hills, MI) |
| Monoclonal-2A12 | 1:500 | DSHB |
| Alexa-phalloidin | 1:500 | Molecular Probes, Invitrogen, Carlsbad, CA |
| Secondary antibodies: | ||
| Alexas 488, 568, and 647 | 1:500 | Molecular Probes, Invitrogen |