Figure 3.

Virtual Northern blot analysis of Praja1 expression using a 5′ Praja1 probe (a) and clone #VIIG5 probe (b). Both blots show strongly increased labeling in the associative learning group A and a graded response across behavioral groups with intermediate induction in the nonassociative training group NA and lowest (two– to threefold) increase of expression in the retrieval group R^A. Praja1 showed four major and several minor bands between 1.4kb and 2.7kb. Clone #VIIG5 hybridized with the 2.7kb, 2.3kb, and in group A also with the 2.0kb Praja1 transcript. However, overall labeling was much weaker than with the 5′ Praja1 probe.