Table 1.
Identification and Expression Levels of Subtraction Products
| Clone #
|
Identity of subtraction product
|
Size of detected transcripts
|
Expression level (relative to U)
|
||
|---|---|---|---|---|---|
| A
|
NA
|
RA
|
|||
| IIIA1 | UDP-galactose transporter related isozyme 1 | 1.0 kb | 1.4 | 1.2 | 1.0 |
| IIID3, IIIE5 | Phosphodiesterase1 | 2.9 kb | 1.5 | 1.7 | 1.0 |
| 2.3 kb* | 1.5 | 1.4 | 1.0 | ||
| IIIF3 | Actin | 2.6 kb§ | 1.4 | 1.1 | 1.1 |
| 1.5 kb | 0.7 | 0.8 | 0.9 | ||
| IVB4 | Proteasome subunit P31 | 1.4 kb | A, NA > RA, U | ||
| 1.1 kb | |||||
| IVH4 | E2-ubiquitin conjugating enzyme | 1.5 kb§ | 1.2 | 1.3 | 0.7 |
| 0.7 kb** | 2.5 | 2.5 | 1.2 | ||
| IVH9 | Neuroligin1 | 5.0 kb | A, NA > RA, U | ||
| 4.8 kb | |||||
| 3.7 kb | A, NA > RA, U | ||||
| 3.5 kb | |||||
| IVH5.2 | Neurocalcin | 3.3 kb** | 1.3 | 1.1 | 1.1 |
| VIA8 | Phosphofructokinase1c | 2.8 kb | 1.3 | 1.2 | 1.0 |
| VIIG5 | Praja1-subtraction product | 2.7 kb*** | 6.5 | 4.9 | 1.6 |
| 2.3 kb*** | 12.1 | 6.5 | 3.2 | ||
| 2.0 kb§ | A, NA > RA, U | ||||
| Praja1-total | 2.7 kb** | 4.9 | 3.7 | 1.6 | |
| 2.3 kb** | 9.8 | 6.2 | 2.7 | ||
| 2.0 kb§ | A > NA > RA, U | ||||
| 1.8 kb* | 10.9 | 8.7 | 3.0 | ||
| 1.4 kb* | 4.8 | 5.2 | 2.2 | ||
| VIIH2 | Transthyretin | 1.1 kb§* | 1.7 | 1.7 | 1.0 |
| 0.8 kb | 1.4 | 1.4 | 1.0 | ||
| VIIIH6 | Aldehyde reductase | 1.5 kb*** | 7.2 | 4.3 | 2.2 |
| 0.7 kb§* | 2.5 | 1.8 | 1.2 | ||
| IXC1 | Protein kinase inhibitor α | 3.6 kb | A, NA > RA, U | ||
| Membrane interacting | 1.6 kb | A > NA, RA, U | |||
| protein of RGS16 | 1.3 kb | ||||
Of 21 clones selected during screening, the 12 listed here could be identified and were further investigated with virtual Northern analysis. The length of cDNA transcripts is largely in agreement with the mRNA sizes reported for the respective molecules; however, some molecules (phosphodiesterase1, neuroligin 1, proteasome subunit P31, and the membrane interacting protein of RGS16) showed doublet transcripts. Expression levels are given in relation to levels in the unshocked group U and are corrected for G3PDH. Proteasome subunit P31, neuroligin1, protein kinase inhibitor α, and the membrane interacting protein of RGS16, as well as some Praja1 transcripts, could not be quantified due to low expression levels; differences in their expression between behavioral groups were therefore judged by visual inspection.
Significant change of expression, P <0.05; **P <0.05; ***P <0.001; §minor band.