Table 4.

RNA-seq results showing the number of reads after the purity filter, the number of reads aligned to our reference database with percent of total number of reads in brackets, and the number of reference sequences the reads mapped to in our database with percentage of total number of sequences in brackets.

	Number of reads (after purity filter)	Number of reads mapped (%)	Number of reference sequences matched (%)
Guppy pred - 1	32,054,094	16,229,906 (51%)	42,501 (73%)
Guppy pred - 2	31,238,411	16,158,014 (51%)	42,256 (72%)
Guppy pred + 1	30,811,092	15,603,608 (51%)	42,072 (72%)
Guppy pred + 2	30,680,881	16,752,768 (55%)	43,099 (74%)
Sailfin molly	29,754,476	12,248,933 (41%)	39,704 (68%)