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. 2011 Jun 13;6(6):e20823. doi: 10.1371/journal.pone.0020823

Figure 1. Restriction Fragment Length Polymorphism Analysis of NYMC X-197 Polymerase and Nucleoprotein Gene Segments.

Figure 1

NYMC X-197 generated from A/Brisbane/11/2010 (H3N2)×A/PR/8/1934 (H1N1) has a 5∶3 gene ratio, the PB1 gene segment was derived from wt virus; PB2, PA and NP gene segments were derived from A/PR/8/1934. A. PB2: lane 1: A/PR/8/1934 undigested; 2: A/PR/8/1934 digested with PvuII; 3: A/Brisbane/11/2010 undigested; 4: A/Brisbane/11/2010 digested with PvuII; 5: NYMC X-197 undigested; 6: NYMC X-197 digested with PvuII. B. PB1: lane 1: A/PR/8/1934 undigested; 2: A/PR/8/1934 digested with PvuII; 3: A/Brisbane/11/2010 undigested; 4: A/Brisbane/11/2010 digested with PvuII; 5: NYMC X-197 undigested; 6: NYMC X-197 digested with PvuII. C. PA: lane 1: A/PR/8/1934 undigested; 2 and 3: A/PR/8/1934 digested with HindIII and XmnI, respectively; 4: A/Brisbane/11/2010 undigested; 5 and 6: A/Brisbane/11/2010 digested with HindIII and XmnI, respectively; 7: NYMC X-197 undigested; 8 and 9: NYMC X-197 digested with HindIII and XmnI, respectively. D. NP: lane 1: A/PR/8/1934 undigested; 2 and 3: A/PR/8/1934 digested with HindIII and XmnI, respectively; 4: A/Brisbane/11/2010 undigested; 5 and 6: A/Brisbane/11/2010 digested with HindIII and XmnI, respectively; 7: NYMC X-197 undigested; 8 and 9: NYMC X-197 digested with HindIII and XmnI, respectively.