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| Gene Segment | Enzyme I | Enzyme II |
| PB2 | PvuII | - |
| PB1 | PvuII | - |
| PA | HindIII | XmnI |
| HA | PvuII | HindIII |
| NP | HindIII | XmnI |
| NA | BsgI | Eco57I |
| M | BsgI | - |
| SmlI* | - | |
| NS | SmlI | XmnI |
Gene segments PB2, PB1 and M were digested by a single enzyme.
*SmlI was used to digest 2009 H1N1pdm M gene segment.
