Table 4. Oligonucleotide Primers used in RT-PCR.

Gene	Primer Sequence	Primer Length (nt)	Annealing Temp. (°C)	Size of Amplicon (∼nt)
PB2	F: AGCGAAAGCAGGTCAATTATATT	23	61	2341
	R: AGTAGAAACAAGGTCGTTTTTAA	23
PB1	F: AGCGAAAGCAGGCAAACCATTTG	23	61	2341
	R: AGTAGAAACAAGGCATTTTTTCA	23
PA	F: AGCGAAAGCAGGTACTGATC	20	61	2233
	R: AGTAGAAACAAGGTACTTTTTTG	23
HA	F: GTTCAGAAAAAGCAGGGG	18	55	1778
	R: AGTAGAAACAAGGGTGTTTT	20
NP	F: AGCAAAAGACAGGGTAGATAATC	23	55	1565
	R: AGTAGAAACAAGGGTATTTTTC	22
NA	F: AGCGAAAGCAGGAGTTTAAAAT	22	55	1413
	R: AGTAGAAACAAGGAGTTTTTTG	22
M	F: AGCGAAAGCAGGTAGATATTGA	22	55	1027
	R: AGTAGAAACAAGGTAGTTTTTT	22
NS	F: AGCAAAAGCAGGGTGACAAAAA	22	55	890
	R: AGTAGAAACAAGGGTGTTTTTT	22

F: forward primers; R: reverse primers used for PCR amplification.