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. 2001 Mar 13;98(7):3837–3842. doi: 10.1073/pnas.061358098

Figure 4.

Figure 4

MH2 domain of Smad3 is required for menin interaction. (a) Schema of full-length menin and its deletion mutants, and Smad3 and its deletion mutant. (b) Structural requirement of menin for the interaction with Smad3. Myc-Smad3 was transfected into COS7 cells with the indicated menin deletion mutants. Total cell extracts were immunoprecipitated with anti-myc antibody followed by SDS/PAGE, immunoblotting, and staining with antibodies against amino- or carboxyl-terminal menin. Expression of myc or menin was monitored. (c) Structural requirement of Smad3 for the interaction with menin. Menin was transfected into COS7 cells with myc-Smad3 or myc-Smad3ΔC mutant. Cell extracts were immunoprecipitated with anti- myc antibodies followed by SDS/PAGE and immunoblotting with anti- menin antibody. Expression of myc or menin was monitored. W, Western blot; IP, immunoprecipitation.