Table 2.
Spot No.a | Protein | Accession Numberb | Molecular Functionc | Reference Organism | Theoretical kDa/pId | Experimental kDa/pIe | Scoref | SCg (%) | Matched/Unmatched queriesh |
---|---|---|---|---|---|---|---|---|---|
Metabolism | |||||||||
91 | Fructokinase-1 | A2WXV8 | ATP binding | Oryza sativa | 34.9/5.1 | 35.8/3.4 | 76 | 20 | 6/59 |
95 | Caffeic acid 3-O-methyltransferase | Q8GU25 | caffeate O-methyltransferase activity | Rosa chinensis | 40.1/5.6 | 43.7/4.4 | 110 | 35 | 12/30 |
129 | Malate dehydrogenase, mitochondrial | P83373 | Oxidoreductase | Fragaria ananassa | 35.8/8.7 | 39.1/7.8 | 74 | 31 | 8/28 |
139 | Glyceraldehyde-3-phosphate dehydrogenase, cytosolic | P25858 | Oxidoreductase | Arabidopsis thaliana | 37.0/6.6 | 40.1/9.3 | 74 | 29 | 9/27 |
141 | Malate dehydrogenase, mitochondrial | P83373 | Oxidoreductase | Fragaria ananassa | 35.8/8.7 | 41.0/9.1 | 65 | 27 | 6/23 |
172 | S-adenosylmethionine synthase 2 | Q9FUZ1 | ATP binding | Brassica juncea | 43.3/5.3 | 43.9/5.9 | 138 | 44 | 15/36 |
173 | S-adenosylmethionine synthase | A4PU48 | ATP binding | Medicago truncatula | 43.7/5.6 | 38.5/6.8 | 178 | 52 | 15/17 |
174 | S-adenosylmethionine synthase | Q8W3Y4 | ATP binding | Phaseolus lunatus | 43.5/5.6 | 41.2/6.7 | 132 | 48 | 15/40 |
Energy | |||||||||
176 | ATP synthase subunit beta, plastid | Q8MBG5 | ATP binding | Cuscuta pentagona | 53.2/5.5 | 53.4/6.7 | 61 | 32 | 10/53 |
Photosynthesis | |||||||||
17 | Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 4OS | Q9T079 | alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity | Arabidopsis thaliana | 90.3/6.1 | 14.2/6.3 | 58 | 25 | 12/66 |
33 | Ribulose bisphosphate carboxylase large chain (Fragment) OS | P28261 | magnesium ion binding | Nypa fruticans | 51.5/6.2 | 22.3/5.1 | 123 | 23 | 13/61 |
35 | Probable granule-bound starch synthase 1, chloroplastic/amyloplastic | Q9MAQ0 | starch synthase activity | Arabidopsis thaliana | 67.5/8.7 | 23.0/4.5 | 60 | 24 | 9/37 |
52 | Ribulose bisphosphate carboxylase large chain (Fragment) | P28391 | magnesium ion binding | Ceratopetalum gummiferum | 51.3/6.2 | 24.0/6.9 | 104 | 22 | 11/63 |
64 | Ribulose-phosphate 3-epimerase, chloroplastic | Q43157 | ribulose-phosphate 3-epimerase activity | Spinacia oleracea | 30.6/8.2 | 27.2/7.9 | 58 | 31 | 6/51 |
82 | Triosephosphate isomerase, chloroplastic | Q9M4S8 | triose-phosphate isomerase activity | Fragaria ananassa | 33.7/7.6 | 31.5/5.2 | 154 | 51 | 16/71 |
90 | Oxygen-evolving enhancer protein 1, chloroplastic | P26320 | calcium ion binding | Solanum tuberosum | 35.6/5.8 | 34.5/4.7 | 98 | 31 | 10/48 |
103 | Coproporphyrinogen-III oxidase, chloroplastic | P35055 | coproporphyrinogen oxidase activity | Glycine max | 43.6/6.7 | 42.2/5.2 | 84 | 31 | 10/32 |
104 | Glutamyl-tRNA reductase 1, chloroplastic | Q42843 | NADP or NADPH binding | Hordeum vulgare | 58.1/8.7 | 42.1/4.9 | 70 | 24 | 11/48 |
Transcription | |||||||||
18 | Homeobox-leucine zipper protein GLABRA 2 OS | P46607 | sequence-specific DNA binding | Arabidopsis thaliana | 83.7/6.1 | 15.0/6.4 | 63 | 31 | 20/90 |
47 | Protein HIRA | Q32SG6 | transcription regulator activity | Zea mays | 106.55/7.8 | 19.7/7.1 | 60 | 22 | 16/90 |
121 | Two-component response regulator ARR9 | O80366 | two-component response regulator activity | Arabidopsis thaliana | 26.2/5.2 | 34.6/8.1 | 58 | 35 | 5/43 |
128 | DNA-directed RNA polymerase subunit beta | P12465 | DNA binding | Chlorella vulgaris | 179.4/9.9 | 36.9/8.0 | 60 | 9 | 11/34 |
149 | B3 domain-containing protein Os07g0679700 | Q6Z3U3 | DNA binding | Oryza sativa | 105.4/6.5 | 53.5/7.9 | 63 | 11 | 9/27 |
168 | Protein HIRA | Q32SG6 | transcription regulator activity | Zea mays | 106.5/7.8 | 41.1/6.8 | 60 | 22 | 16/90 |
Protein synthesis | |||||||||
28 | Eukaryotic translation initiation factor 5A-2 OS | Q945F4 | translation initiation factor activity | Medicago sativa | 17.5/5.4 | 17.3/4.7 | 74 | 26 | 5/39 |
37 | Eukaryotic translation initiation factor 5A | Q9AXQ7 | translation initiation factor activity | Dianthus caryophyllus | 17.6/5.6 | 20.3/5.9 | 88 | 45 | 7/28 |
45 | Glutathione gamma-glutamylcysteinyltransferase 2 | Q9ZWB7 | acyltransferase activity | Arabidopsis thaliana | 52.3/6.6 | 16.4/6.6 | 57 | 15 | 8/32 |
66 | Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic | P21357 | 3-deoxy-7-phosphoheptulonate synthase activity | Solanum tuberosum | 60.0/8.9 | 27.9/7.3 | 62 | 31 | 11/71 |
87 | Molybdenum cofactor sulfurase | Q655R6 | lyase activity | Oryza sativa | 92.9/7.1 | 27.2/5.2 | 60 | 12 | 6/22 |
136 | Probable beta-1,3-galactosyltransferase 18 | Q8RX55 | galactosyltransferase activity | Arabidopsis thaliana | 77.9/8.7 | 35.1/9.1 | 66 | 17 | 7/27 |
Protein folding, degradation and assembly | |||||||||
148 | Anaphase-promoting complex subunit 2 | Q8H1U5 | ubiquitin protein ligase binding | Arabidopsis thaliana | 98.4/4.8 | 43.5/7.9 | 64 | 12 | 7/20 |
153 | U-box domain-containing protein 34 | Q8S8S7 | ubiquitin-protein ligase activity | Arabidopsis thaliana | 91.7/9.1 | 39.6/6.9 | 63 | 18 | 12/51 |
171 | Ubiquitin carboxyl-terminal hydrolase 6 | Q949Y0 | ubiquitin-specific protease activity | Arabidopsis thaliana | 54.0/5.8 | 41.6/5.8 | 58 | 19 | 8/35 |
175 | U-box domain-containing protein 34 | Q8S8S7 | ubiquitin-protein ligase activity | Arabidopsis thaliana | 91.7/9.1 | 44.7/6.2 | 58 | 19 | 12/67 |
Transport | |||||||||
24 | Magnesium transporter MRS2-8 OS | Q8H1G7 | metal ion transmembrane transporter activity | Arabidopsis thaliana | 43.1/5.3 | 13.6/4.4 | 58 | 22 | 6/34 |
54 | Putative copper-transporting ATPase 3 | Q9SH30 | ATP binding | Arabidopsis thaliana | 109.0/6.0 | 29.0/8.0 | 65 | 18 | 14/48 |
Stress Related | |||||||||
4 | Ninja-family protein AFP4 OS | Q9S7Z2 | protein binding | Arabidopsis thaliana | 35.6/8.5 | 7.1/5.0 | 57 | 40 | 9/70 |
9 | Glutathione S-transferase 6 | Q96266 | glutathione binding | Arabidopsis thaliana | 29.2/8.5 | 11.9/6.2 | 58 | 25 | 5/46 |
20 | Annexin D6 OS | Q9LX08 | calcium ion binding | Arabidopsis thaliana | 36.6/7.7 | 14.0/5.1 | 62 | 20 | 7/39 |
41 | Monodehydroascorbate reductase, chloroplastic | P92947 | monodehydroascorbate reductase (NADH) activity | Arabidopsis thaliana | 53.5/8.1 | 16.2/5.7 | 63 | 26 | 8/76 |
154 | Monodehydroascorbate reductase | Q40977 | monodehydroascorbate reductase (NADH) activity | Pisum sativum | 47.3/5.8 | 43.5/6.8 | 58 | 22 | 7/31 |
Development | |||||||||
2 | Pentatricopeptide repeat-containing protein At3g06430, chloroplastic OS | Q9SQU6 | N/A | Arabidopsis thaliana | 56.2/7.8 | 9.5/6.7 | 58 | 25 | 11/59 |
11 | Protein BRUSHY 1 | Q6Q4D0 | protein binding | Arabidopsis thaliana | 148.8/5.5 | 12.0/5.9 | 63 | 24 | 21/71 |
38 | Protein PAIR1 | Q75RY2 | Oryza sativa | 53.8/9.8 | 17.2/5.5 | 66 | 19 | 10/34 | |
68 | 1-aminocyclopropane-1-carboxylate synthase 7 | Q9STR4 | 1-aminocyclopropane-1-carboxylate synthase activity | Arabidopsis thaliana | 51.0/5.9 | 26.2/6.3 | 57 | 19 | 10/72 |
Unknown | |||||||||
12 | Probable protein ABIL5 OS | Q5JMF2 | N/A | Oryza sativa | 28.3/8.3 | 15.2/7.6 | 59 | 40 | 7/42 |
16 | Pentatricopeptide repeat-containing protein At4g26800 OS | Q9SZ20 | N/A | Arabidopsis thaliana | 58.4/9.1 | 16.1/6.8 | 64 | 32 | 12/49 |
31 | Putative F-box protein At1g20795 OS | Q9LM74 | N/A | Arabidopsis thaliana | 48.5/8.6 | 20.3/5.1 | 60 | 21 | 8/55 |
48 | Probable protein ABIL5 OS | Q5JMF2 | N/A | Oryza sativa | 28.2/8.2 | 21.5/6.9 | 59 | 40 | 7/42 |
67 | BRCT domain-containing protein At4g02110 | O04251 | N/A | Arabidopsis thaliana | 142.5/8.4 | 28.4/7.5 | 59 | 19 | 18/80 |
108 | Thylakoid lumenal 15.0 kDa protein 2, chloroplastic | Q9LVV5 | N/A | Arabidopsis thaliana | 24.7/5.7 | 37.8/5.8 | 68 | 26 | 7/37 |
113 | Pentatricopeptide repeat-containing protein At2g01860 | Q5XET4 | N/A | Arabidopsis thaliana | 56.0/9.2 | 36.1/6.8 | 59 | 39 | 15/95 |
aSpot number as in Figure 2. bSwissprot database accession number. cMolecular functions were inferred from those reported in the UniProt database. dTheoretical molecular mass and isoelectric point. eExperimental molecular mass and isoelectric point. fMascot score. gSequence coverage. hNumber of matched and unmatched peptides.