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. 2011 May 14;9:26. doi: 10.1186/1477-5956-9-26

Table 2.

Identified proteins from the strawberry stolon I-2 internode

Spot No.a Protein Accession Numberb Molecular Functionc Reference Organism Theoretical kDa/pId Experimental kDa/pIe Scoref SCg (%) Matched/Unmatched queriesh
Metabolism
91 Fructokinase-1 A2WXV8 ATP binding Oryza sativa 34.9/5.1 35.8/3.4 76 20 6/59
95 Caffeic acid 3-O-methyltransferase Q8GU25 caffeate O-methyltransferase activity Rosa chinensis 40.1/5.6 43.7/4.4 110 35 12/30
129 Malate dehydrogenase, mitochondrial P83373 Oxidoreductase Fragaria ananassa 35.8/8.7 39.1/7.8 74 31 8/28
139 Glyceraldehyde-3-phosphate dehydrogenase, cytosolic P25858 Oxidoreductase Arabidopsis thaliana 37.0/6.6 40.1/9.3 74 29 9/27
141 Malate dehydrogenase, mitochondrial P83373 Oxidoreductase Fragaria ananassa 35.8/8.7 41.0/9.1 65 27 6/23
172 S-adenosylmethionine synthase 2 Q9FUZ1 ATP binding Brassica juncea 43.3/5.3 43.9/5.9 138 44 15/36
173 S-adenosylmethionine synthase A4PU48 ATP binding Medicago truncatula 43.7/5.6 38.5/6.8 178 52 15/17
174 S-adenosylmethionine synthase Q8W3Y4 ATP binding Phaseolus lunatus 43.5/5.6 41.2/6.7 132 48 15/40
Energy
176 ATP synthase subunit beta, plastid Q8MBG5 ATP binding Cuscuta pentagona 53.2/5.5 53.4/6.7 61 32 10/53
Photosynthesis
17 Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 4OS Q9T079 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Arabidopsis thaliana 90.3/6.1 14.2/6.3 58 25 12/66
33 Ribulose bisphosphate carboxylase large chain (Fragment) OS P28261 magnesium ion binding Nypa fruticans 51.5/6.2 22.3/5.1 123 23 13/61
35 Probable granule-bound starch synthase 1, chloroplastic/amyloplastic Q9MAQ0 starch synthase activity Arabidopsis thaliana 67.5/8.7 23.0/4.5 60 24 9/37
52 Ribulose bisphosphate carboxylase large chain (Fragment) P28391 magnesium ion binding Ceratopetalum gummiferum 51.3/6.2 24.0/6.9 104 22 11/63
64 Ribulose-phosphate 3-epimerase, chloroplastic Q43157 ribulose-phosphate 3-epimerase activity Spinacia oleracea 30.6/8.2 27.2/7.9 58 31 6/51
82 Triosephosphate isomerase, chloroplastic Q9M4S8 triose-phosphate isomerase activity Fragaria ananassa 33.7/7.6 31.5/5.2 154 51 16/71
90 Oxygen-evolving enhancer protein 1, chloroplastic P26320 calcium ion binding Solanum tuberosum 35.6/5.8 34.5/4.7 98 31 10/48
103 Coproporphyrinogen-III oxidase, chloroplastic P35055 coproporphyrinogen oxidase activity Glycine max 43.6/6.7 42.2/5.2 84 31 10/32
104 Glutamyl-tRNA reductase 1, chloroplastic Q42843 NADP or NADPH binding Hordeum vulgare 58.1/8.7 42.1/4.9 70 24 11/48
Transcription
18 Homeobox-leucine zipper protein GLABRA 2 OS P46607 sequence-specific DNA binding Arabidopsis thaliana 83.7/6.1 15.0/6.4 63 31 20/90
47 Protein HIRA Q32SG6 transcription regulator activity Zea mays 106.55/7.8 19.7/7.1 60 22 16/90
121 Two-component response regulator ARR9 O80366 two-component response regulator activity Arabidopsis thaliana 26.2/5.2 34.6/8.1 58 35 5/43
128 DNA-directed RNA polymerase subunit beta P12465 DNA binding Chlorella vulgaris 179.4/9.9 36.9/8.0 60 9 11/34
149 B3 domain-containing protein Os07g0679700 Q6Z3U3 DNA binding Oryza sativa 105.4/6.5 53.5/7.9 63 11 9/27
168 Protein HIRA Q32SG6 transcription regulator activity Zea mays 106.5/7.8 41.1/6.8 60 22 16/90
Protein synthesis
28 Eukaryotic translation initiation factor 5A-2 OS Q945F4 translation initiation factor activity Medicago sativa 17.5/5.4 17.3/4.7 74 26 5/39
37 Eukaryotic translation initiation factor 5A Q9AXQ7 translation initiation factor activity Dianthus caryophyllus 17.6/5.6 20.3/5.9 88 45 7/28
45 Glutathione gamma-glutamylcysteinyltransferase 2 Q9ZWB7 acyltransferase activity Arabidopsis thaliana 52.3/6.6 16.4/6.6 57 15 8/32
66 Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic P21357 3-deoxy-7-phosphoheptulonate synthase activity Solanum tuberosum 60.0/8.9 27.9/7.3 62 31 11/71
87 Molybdenum cofactor sulfurase Q655R6 lyase activity Oryza sativa 92.9/7.1 27.2/5.2 60 12 6/22
136 Probable beta-1,3-galactosyltransferase 18 Q8RX55 galactosyltransferase activity Arabidopsis thaliana 77.9/8.7 35.1/9.1 66 17 7/27
Protein folding, degradation and assembly
148 Anaphase-promoting complex subunit 2 Q8H1U5 ubiquitin protein ligase binding Arabidopsis thaliana 98.4/4.8 43.5/7.9 64 12 7/20
153 U-box domain-containing protein 34 Q8S8S7 ubiquitin-protein ligase activity Arabidopsis thaliana 91.7/9.1 39.6/6.9 63 18 12/51
171 Ubiquitin carboxyl-terminal hydrolase 6 Q949Y0 ubiquitin-specific protease activity Arabidopsis thaliana 54.0/5.8 41.6/5.8 58 19 8/35
175 U-box domain-containing protein 34 Q8S8S7 ubiquitin-protein ligase activity Arabidopsis thaliana 91.7/9.1 44.7/6.2 58 19 12/67
Transport
24 Magnesium transporter MRS2-8 OS Q8H1G7 metal ion transmembrane transporter activity Arabidopsis thaliana 43.1/5.3 13.6/4.4 58 22 6/34
54 Putative copper-transporting ATPase 3 Q9SH30 ATP binding Arabidopsis thaliana 109.0/6.0 29.0/8.0 65 18 14/48
Stress Related
4 Ninja-family protein AFP4 OS Q9S7Z2 protein binding Arabidopsis thaliana 35.6/8.5 7.1/5.0 57 40 9/70
9 Glutathione S-transferase 6 Q96266 glutathione binding Arabidopsis thaliana 29.2/8.5 11.9/6.2 58 25 5/46
20 Annexin D6 OS Q9LX08 calcium ion binding Arabidopsis thaliana 36.6/7.7 14.0/5.1 62 20 7/39
41 Monodehydroascorbate reductase, chloroplastic P92947 monodehydroascorbate reductase (NADH) activity Arabidopsis thaliana 53.5/8.1 16.2/5.7 63 26 8/76
154 Monodehydroascorbate reductase Q40977 monodehydroascorbate reductase (NADH) activity Pisum sativum 47.3/5.8 43.5/6.8 58 22 7/31
Development
2 Pentatricopeptide repeat-containing protein At3g06430, chloroplastic OS Q9SQU6 N/A Arabidopsis thaliana 56.2/7.8 9.5/6.7 58 25 11/59
11 Protein BRUSHY 1 Q6Q4D0 protein binding Arabidopsis thaliana 148.8/5.5 12.0/5.9 63 24 21/71
38 Protein PAIR1 Q75RY2 Oryza sativa 53.8/9.8 17.2/5.5 66 19 10/34
68 1-aminocyclopropane-1-carboxylate synthase 7 Q9STR4 1-aminocyclopropane-1-carboxylate synthase activity Arabidopsis thaliana 51.0/5.9 26.2/6.3 57 19 10/72
Unknown
12 Probable protein ABIL5 OS Q5JMF2 N/A Oryza sativa 28.3/8.3 15.2/7.6 59 40 7/42
16 Pentatricopeptide repeat-containing protein At4g26800 OS Q9SZ20 N/A Arabidopsis thaliana 58.4/9.1 16.1/6.8 64 32 12/49
31 Putative F-box protein At1g20795 OS Q9LM74 N/A Arabidopsis thaliana 48.5/8.6 20.3/5.1 60 21 8/55
48 Probable protein ABIL5 OS Q5JMF2 N/A Oryza sativa 28.2/8.2 21.5/6.9 59 40 7/42
67 BRCT domain-containing protein At4g02110 O04251 N/A Arabidopsis thaliana 142.5/8.4 28.4/7.5 59 19 18/80
108 Thylakoid lumenal 15.0 kDa protein 2, chloroplastic Q9LVV5 N/A Arabidopsis thaliana 24.7/5.7 37.8/5.8 68 26 7/37
113 Pentatricopeptide repeat-containing protein At2g01860 Q5XET4 N/A Arabidopsis thaliana 56.0/9.2 36.1/6.8 59 39 15/95

aSpot number as in Figure 2. bSwissprot database accession number. cMolecular functions were inferred from those reported in the UniProt database. dTheoretical molecular mass and isoelectric point. eExperimental molecular mass and isoelectric point. fMascot score. gSequence coverage. hNumber of matched and unmatched peptides.