Table 2.
Primers useda
| Primer | Sequence |
|---|---|
| DF1 | 5' AAGCT(A/C/G/T)GG(A/C/G/T)G(C/T)(A/C/G/T)CCT T(C/T)(C/T)TATTGTTT(C/T)CACGAC 3' |
| DR2 | 5' TCCTCC(A/C/G/T)TGCTG(C/T)(T/A)(T/A)GCCGCC(A/C/G/T)CC(A/C/G/T)CG(A/C/G/T)AG(A/G)AT 3' |
| DF2 | 5' GGT(A/G)T(A/C/G/T)AA(A/G)CT(A/C/G/T)CTCTGGGG(A/C/G/T)CAGGCCAA(C/T)CT(A/C/G/T)TT(C/T) 3' |
| DR1 | 5' (A/G)TG(C/T)TTCTGGGG(C/T)TC(C/T)TGCGGCTTGGGCTC (A/C/G/T)A(T/G) 3' |
| pRSETBF | 5' GGTGGACAGCAAATGGGTCGG 3' |
| pRSETBR | 5' GGGCTTTGTTAGCAGCCGGATC 3' |
| XYM1F | 5' CCTGGATCCATGAGCGTTGTTCTTGGCGACAAAG 3' |
| XYM1R | 5' TGGGTCGACTTACCGGATCCACCGGTTCATAATG 3' |
| XYM2F | 5'CAGGAATTCATGAAACTTACCGTAGGAGACAAGG 3' |
| XYM2R | 5' TGGGTCGACTTAAATAAACCTGCTAATCAAATTCTCAATATAC 3' |
aRestriction sites, when present, are underlined.