Table 3.
Reported specific activity and maximum specific growth rate for XI genes cloned in Saccharomyces cerevisiaea
| Source of XI | Activity (U/mg protein) | Aerobic growth rate on xylose (hour-1) | Source | Identity with xym1 XI | Identity with xym2 XI | Identity with Piromyces XI |
|---|---|---|---|---|---|---|
| Metagenome xym1b | 0.33 ± 0.05 | 0.02 | This study | 100% | 67% | 61% |
| Metagenome xym2b | 0.20 ± 0.04 | 0.02 | This study | 67% | 100% | 63% |
| Piromyces sp.b,c | 0.35 | 0.07 | This study | 61% | 63% | 100% |
| Piromyces sp. | 0.3 to 1.1 | 0.22 | [18] | 61% | 63% | 100% |
| Piromyces sp.c | 0.5 to 0.8 | 0.02 | [17] | 61% | 63% | 100% |
| Piromyces sp.c | 0.0538 | 0.056 | [16] | 61% | 63% | 100% |
| Orpinomyces sp. | 1.6 | 0.01 | [15] | 61% | 61% | 94% |
| Xanthomonas campestris | 0.016 | 0.02 | [44] | 64% | 63% | 61% |
| Bacteroides thetaiotaomicron | 0.14 | n.m. | [45] | 61% | 64% | 83% |
| Clostridium phytofermentansc | 0.0344 | 0.057 | [16] | 54% | 52% | 54% |
| Clostridium thermosulfurogenes | n.m. | n.d. | [46] | 51% | 49% | 49% |
| Escherichia coli | n.m. | n.d. | [19] | 50% | 52% | 48% |
| Bacillus subtilis | n.m. | n.m. | [47] | 48% | 48% | 47% |
| Lactobacillus pentosus | n.d. | n.d. | [47] | 48% | 47% | 46% |
| Thermus thermophilus | 0.0007 to 0.012 | n.m. | [48] | 31% | 30% | 29% |
an.m., not measured; n.d., not detected. Sequence identity with xym1, xym2 and Piromyces XI gene is also given at the protein level. bGlucose-grown cells. cCodon-optimised gene.